A Conserved Carboxylesterase Inhibits Tobacco mosaic virus (TMV) Accumulation in Nicotiana benthamiana Plants

A Conserved Carboxylesterase Inhibits Tobacco mosaic virus (TMV) Accumulation in Nicotiana benthamiana Plants

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A carboxylesterase (CXE) or carboxylic-ester hydrolase is an enzyme that catalyzes carboxylic ester and water into alcohol and carboxylate.In here plants, CXEs have been implicated in defense, development, and secondary metabolism.We discovered a new CXE gene in Nicotiana benthamiana that is related to virus resistance.The transcriptional level of NbCXE expression was significantly increased after Tobacco mosaic virus (TMV) infection.

Transient over-expression of NbCXE inhibited TMV accumulation in N.benthamiana plants.Conversely, when the NbCXE gene was silenced with a Tobacco rattle virus (TRV)-based gene silencing system, TMV RNA accumulation was increased in NbCXE-silenced plants after infection.NbCXE protein was shown to interact with TMV coat protein echofix spring reverb (CP) in vitro.

Additionally, the expressions of host defense-related genes were increased in transient NbCXE-overexpressed plants but decreased in NbCXE silenced N.benthamiana plants.In summary, our study showed that NbCXE is a novel resistance-related gene involved in host defense responses against TMV infection.